Composite
Part:BBa_K3365053
Designed by: Zou Aixuan Group: iGEM20_SJTU-BioX-Shanghai (2020-10-22)
RFP with double terminator
This part is used as a reporter for detecting whether dCas9-ωis on-target. We integrated this part downstream of transcription activating unit. If dCas9-ω is binding to target sequence, this part will be activated to express and the cell then emit red fluorescence, allowing us to know that dCas9-ωis literally on-target. As we plan to put the potential off-target site downstream of this part, we added a terminator to RFP so that the expression of this part can stop before next off-target reporting site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 700
Illegal XhoI site found at 691 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |